Description
Principle of the Assay: The Human Hemopexin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Hemopexin in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human Hemopexin coated on a 96-well plate. Standards and samples are pipetted into the wells and Hemopexin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Hemopexin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Hemopexin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.